Electroporation of Kluyveromyces marxianus and -D-galactosidase Extraction
نویسندگان
چکیده
Nowadays electroporation is well established as a method for increasing the permeability of biological membranes aiming to include some kind of molecule inside cells (anticancer drugs, for example) or to extract molecules from cells (enzymes, DNA, etc.). The permeabilized cells show a distribution of hydrophilic pores with diameters of several nanometers in the regions where the induced transmembrane potential rises above a threshold value from 200 mV to 1 V (Hibino et al, 1993; Teissie & Rols, 1993). The pore density and their diameters are dependent on the stimulation conditions: field strength, waveform and duration of stimulus (Weaver & Chizmadzhev, 1996; Teissié et al, 2005; Miklavčič & Puc, 2006; Chen et al, 2006)
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